WP2 has for main objectives to set-up, optimize and perform validation of the zebrafish obesogenic test (ZOT) and other zebrafish-based bioassays related to NAFLD, with the aim to screen and identify endocrine disruptors. The identification of potential exposure/pathological omics signatures of obesity and liver pathologies using zebrafish as a model and analytical chemistry analyses data will be provided to produce exploitable data for modeling approaches.